Objective To analyze the differential expression of genes in postpartum rat animal model by RNA-Seq and characteristics of biological mechanism. Method 9 10-week-old female SD rats were divided into Y0, Y1 and Y2 group randomly, with 3 in each group. RNA was extracted from the abdominal aorta blood of postpartum rats, and transcriptome sequencing was performed using RNA Seq technology. The differentially screened genes were subjected to GO and KEGG bioinformatics analysis. Result Compared Y0, there were 1 222 significantly different genes in both Y1 and Y2. GO analysis showed that significantly different genes were mainly expressed in biological processes related to immunity, oxidative stress and metabolism. KEGG analysis showed that different genes were mainly related to the signal pathways of immune system, signal transduction, signal molecules and interactions, cell growth and death, transportation and catabolism. Conclusion Postpartum rat could simulate the changes of biological function of human postpartum condition as an animal model, could used to observe the impact of pregnancy and childbirth on biological function of the body and explore effective intervention measures.

pregnancy and childbirth, animal model, RNA-sequencing

目的 通过转录组学测序技术( RNA-Seq) 分析孕产大鼠动物模型基因差异性表达,并分析生物机制特征。方法 将 9 只 10 周龄雌性 SD 大鼠按照随机数字表法分为非孕产组(Y0) 、一次孕产组( Y1) 和二次孕产组( Y2) ,每组 3 只。 于产后取大鼠腹主动脉血提取 RNA,采用 RNA-Seq 技术进行转录组学测序,将筛选的差异基因进行 GO和 KEGG 生物信息学分析。 结果 与非孕产大鼠相比,二次孕产与一次孕产大鼠共同差异表达 1 222 个基因。 GO分析发现差异基因主要集中在与免疫、氧化应激、代谢相关的各种生物学过程中,KEGG 分析发现差异基因主要与免疫系统、信号传导、信号分子和相互作用、细胞生长和死亡、运输和分解代谢等信号通路相关。 结论 产后大鼠能模拟人体孕产后生物机能变化情况,可以作为动物模型用于观察孕产对机体生物功能产生的影响,探索有效预防干预手段。

孕产, 动物模型, RNA 测序